Standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software. Bd facsdiva software quick reference guide for bd facscanto. This guide contains instructions for using bd facsdiva software version 6. Becton dickinson bd facsdiva software is a technology used for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows in a laboratory and healthcare setting. Bd lsr ii configuration upgrade if you upgrade your bd lsr ii cytometer with additional lasers or special order detector arrays, you will need to run the ica software to update the bd facsdiva database for your instrument. Cytometry diva 8 stand alone on windows 64 bit computer. Bd facsdiva software provides new features to help users integrate flow systems into new application areas, including index sorting for stem cell and singlecell applications, as well as automation protocols for highthroughput and robotic laboratories. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. Cytometry facsdiva questions and getting started in flow. Bd biosciences is not liable for any claims related to or resulting from buyerusers failure to install and maintain virus protection. Catalog number 641609 flexible data acquistion and analysis package specifically designed for digitalbased flow cytometers. The browser lists folders, experiments, and experimental elements in a hierarchical view, provides an interface for setting up experiments, and contains a current tube pointerc indicating the tube for which acquisition or analysis data will be shown. Tm, use the pe channel for reporter and the apc channel for beads classification. Open bd facsdiva software and use for analysis as normal if you have any questions feel free to.
Setup procedure for bd facsariatm, facscantotm and lsr series. During this process, each beads median fluorescence intensity and robust coefficient of variation are measured in all fluorescence detectors. The process of digitizing and sampling is occurring continuously. Reading facsdiva data files documentation just another. Computer and software login to log into the computer, use lsrii as the user name and lsrii as the password. Bd biosciences, a segment of bd becton, dickinson and company, today announced the introduction of bd facsdiva 6. This problem can occur on bd facsdiva versions b 4 and b 4 when combined with a specific computer system configuration. Cytometry win upgrades on diva computers edited subject. References flowjo software flowjo is a software package for analyzing flow cytometry data. Bd facsdiva software quick reference guide for the bd lsr ii.
Open bd facsdiva software and use for analysis as normal if you have any questions feel free to contact me. Page 2 of 5 red laser 640nm page 3 of 5 violet laser 405nm page 4 of 5 yelgreen. Cells were sorted on high purity setting at no more than 5000 eventss using a 70 m nozzle at 55 psi with a 90 hz. This video will show you how to install bd facsdiva 8. Dec 30, 2017 this video will show you how to install bd facsdiva 8. During this process, each beads median fluorescence intensity and robust coefficient of variation are measured in. In the login window, scroll down through the list of user names to locate yours. Before starting your daily workflow, ensure that your labs software administrator has performed all the necessary tasks. Verify that the hts doors are closed and perform a fluidics startup. Rightclick the original file and choose properties 5.
Linear plots have tickmarks at 0, 10,000, 20,000, and so on. Setup procedure for bd facsariatm, facscantotm and lsr. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. Interference there are no known incompatibilities with bd facscanto clinical. Using application settings to standardize flow cytometry. In the context of similarity of counts obtained between the most widely used and conventional 4 color system, facscalibur, cell quest pro, and facsdiva, and the fact that facsdiva has more options for manual gating, our data suggests that facsdiva software should be preferred over the facscanto software for 6. Workflow overview the following figure shows the steps for daily workflow using bd facsdiva software. The bd protocol discusses what to do in these instances.
The software uses a database server software to manage the flow. Page 41 controls chapter of the bd facsdiva software reference manual for details. The software runs on a windows2000 computer, macintosh systems are no longer available. Bd cytometer setup and tracking application guide trigenotoul. Expand the specimen and make the tube active by clicking the arrow next to the tube. For an indepth description of software components not described in this chapter, refer to the bd facsdiva software reference manual. Bd facsdiva software overview this chapter describes the bd facsdiva software features necessary to operate the bd high throughput sampler with the bd lsr ii, bd facscanto, and bd facscanto ii flow cytometers. Refer to new features in getting started with bd facsdiva software for details. Regarding this runtime module, you are prohibited from.
Bd facsdiva software contains vxworks as embedded software runtime module. The raw pulse height value is a number between 0 and 16,384 14 bits. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments. This automated package will help scientists attain the best performance and most consistent data from bd flow cytometry systems. This problem has not been reproduced in other computer systems or in other bd facsdiva software versions distributed by bd biosciences. The runtime module was developed by a third party and we are obligated to. The cytometer setup and tracking features in bd facsdiva v6. Bd facsdiva tm software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory. Importing bd facsdiva and accuri experiments youtube. History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4. This automated package will help scientists attain the best performance and most consistent data from bd flow cytometry systems used for important basic and clinical research. Standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software step 2 after determining and setting the minimum acceptable voltages, verify that the dynamic range is appropriate and that highly positive cell populations still. A 1004 updated software terminology and screen shots for bd facsdiva software version 4.
History revision date change made 338642 1004 initial release 640756 306 updated to include bd facsdiva software, version 5. Ellen meinelt, mervi reunanen, mark edinger, maria jaimes, alan stall, dennis sasaki, joe trotter. Administrative tasks bd facsdiva software v6 with sample preppers. Under attributes, unclick readonly, then click ok 6. Height is the maximum value of all data points in a given channel within the window gate. Bd facsdiva software overview selfpaced data display in bd facsdiva software selfpaced using the worksheet window in bd facsdiva software selfpaced. Using templates in bd facsdiva software selfpaced bd facsvia online training program. Why waste effort recreating your work, when you can g. Some of the older versions transposed the compensation matrix. This part of the guide tmapplies to bd digital flow cytometers using facsdiva software version 6.
Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Feb 12, 2015 importers allow you to use your plots and gates from acquisition as a starting point for your analysis. The runtime module was developed by a third party and we are obligated to notify our customers about the limitations for use. Bd lsr ii configuration upgrade if you upgrade your bd lsr ii cytometer with additional lasers or special order detector arrays, you will need to run the ica software to update the. Only the us english version of the microsoft windows xp operating. The new digital acquisition platform introduces a complete new software concept from bd. Aug 31, 2017 download sentinel protection installer v7. Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis. Bd facsdiva software resources software bd biosciences.
The cytometer setup and tracking workflow uses cytometer setup and tracking beads and bd facsdiva 6. A 506 updated software terminology and screen shots for bd facsdiva software version 5. The following figure shows the steps for daily workflow using bd facsdiva software. Ddx4positive and negative cells, for both human ovarian cells and transfected hek 293 t cells, were sorted using an aria iiu cytometer equipped with a 488 nm laser, running bd facsdiva v6 software bd biosciences, oxford, uk.
Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. Set up will ask what cytometer is going to be connected, select whichever cytometer you use most often. Older bd facsdiva versions you may sometimes wish to export as experiment. Across multiple flow cytometers using bd facsdiva version 6 software. Importers allow you to use your plots and gates from acquisition as a starting point for your analysis. Class 2 device recall bd biosciences facsdiva software. The system has been tested with the fcs files created by bd cellquest, bd facsdiva software version 5 and beckmancoulter quanta software. Open sybase sql anywhere folder and run sqlany12 installer 5. Refer to the information supplied by the manufacturer for applicationspecific limitations.
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